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nicd fragment  (Addgene inc)


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    Structured Review

    Addgene inc nicd fragment
    Nicd Fragment, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nicd+fragment/TetO-FUW-NICD+(Plasmid+%2361540)/pm37042949-36-7-11
    Average 92 stars, based on 9 article reviews
    nicd fragment - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Over Expression:

    Article Title: Loss of p53 enhances the tumor-initiating potential and drug resistance of clonogenic multiple myeloma cells.
    Article Snippet: For short hairpin RNA (shRNA) knockdown (KD) studies, shRNA sequences (supplemental Table 2) were selected based on The RNAi Consortium library (Sigma) and cloned into the pLKO.1-blast or pLKO.1-mCherry lentiviral vectors (Addgene). shRNA KD cells 3552 CHANG et al were selected using blasticidin or sorted using BD FACS Aria (BD Biosciences). .. For Notch intracellular domain (NICD) overexpression, the NICD fragment (from TetO-FUW-NICD; Addgene) was cloned into the pINDUCER21 (Addgene) lentiviral vector, then transfected into MM.1S p53-KO cells. .. Green fluorescent protein (GFP)–positive cells were sorted by BD FACS Aria.

    Article Title: Loss of p 53 enhances the tumor-initiating potential and drug resistance of clonogenic multiple myeloma cells
    Article Snippet: For short hairpin RNA (shRNA) knockdown (KD) studies, shRNA sequences ( ) were selected based on The RNAi Consortium library (Sigma) and cloned into the pLKO.1-blast or pLKO.1-mCherry lentiviral vectors (Addgene). shRNA KD cells were selected using blasticidin or sorted using BD FACS Aria (BD Biosciences). .. For Notch intracellular domain (NICD) overexpression, the NICD fragment (from TetO-FUW-NICD; Addgene) was cloned into the pINDUCER21 (Addgene) lentiviral vector, then transfected into MM.1S p 53-KO cells. .. Green fluorescent protein (GFP)–positive cells were sorted by BD FACS Aria.

    Clone Assay:

    Article Title: Loss of p53 enhances the tumor-initiating potential and drug resistance of clonogenic multiple myeloma cells.
    Article Snippet: For short hairpin RNA (shRNA) knockdown (KD) studies, shRNA sequences (supplemental Table 2) were selected based on The RNAi Consortium library (Sigma) and cloned into the pLKO.1-blast or pLKO.1-mCherry lentiviral vectors (Addgene). shRNA KD cells 3552 CHANG et al were selected using blasticidin or sorted using BD FACS Aria (BD Biosciences). .. For Notch intracellular domain (NICD) overexpression, the NICD fragment (from TetO-FUW-NICD; Addgene) was cloned into the pINDUCER21 (Addgene) lentiviral vector, then transfected into MM.1S p53-KO cells. .. Green fluorescent protein (GFP)–positive cells were sorted by BD FACS Aria.

    Article Title: Loss of p 53 enhances the tumor-initiating potential and drug resistance of clonogenic multiple myeloma cells
    Article Snippet: For short hairpin RNA (shRNA) knockdown (KD) studies, shRNA sequences ( ) were selected based on The RNAi Consortium library (Sigma) and cloned into the pLKO.1-blast or pLKO.1-mCherry lentiviral vectors (Addgene). shRNA KD cells were selected using blasticidin or sorted using BD FACS Aria (BD Biosciences). .. For Notch intracellular domain (NICD) overexpression, the NICD fragment (from TetO-FUW-NICD; Addgene) was cloned into the pINDUCER21 (Addgene) lentiviral vector, then transfected into MM.1S p 53-KO cells. .. Green fluorescent protein (GFP)–positive cells were sorted by BD FACS Aria.

    Transfection:

    Article Title: Loss of p53 enhances the tumor-initiating potential and drug resistance of clonogenic multiple myeloma cells.
    Article Snippet: For short hairpin RNA (shRNA) knockdown (KD) studies, shRNA sequences (supplemental Table 2) were selected based on The RNAi Consortium library (Sigma) and cloned into the pLKO.1-blast or pLKO.1-mCherry lentiviral vectors (Addgene). shRNA KD cells 3552 CHANG et al were selected using blasticidin or sorted using BD FACS Aria (BD Biosciences). .. For Notch intracellular domain (NICD) overexpression, the NICD fragment (from TetO-FUW-NICD; Addgene) was cloned into the pINDUCER21 (Addgene) lentiviral vector, then transfected into MM.1S p53-KO cells. .. Green fluorescent protein (GFP)–positive cells were sorted by BD FACS Aria.

    Article Title: Loss of p 53 enhances the tumor-initiating potential and drug resistance of clonogenic multiple myeloma cells
    Article Snippet: For short hairpin RNA (shRNA) knockdown (KD) studies, shRNA sequences ( ) were selected based on The RNAi Consortium library (Sigma) and cloned into the pLKO.1-blast or pLKO.1-mCherry lentiviral vectors (Addgene). shRNA KD cells were selected using blasticidin or sorted using BD FACS Aria (BD Biosciences). .. For Notch intracellular domain (NICD) overexpression, the NICD fragment (from TetO-FUW-NICD; Addgene) was cloned into the pINDUCER21 (Addgene) lentiviral vector, then transfected into MM.1S p 53-KO cells. .. Green fluorescent protein (GFP)–positive cells were sorted by BD FACS Aria.



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    nicd fragment  (Addgene inc)
    92
    Addgene inc nicd fragment
    Nicd Fragment, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nicd+fragment/TetO-FUW-NICD+(Plasmid+%2361540)/pm37042949-36-7-11
    Average 92 stars, based on 1 article reviews
    nicd fragment - by Bioz Stars, 2026-09
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    teto-fuw-nicd fragment  (Addgene inc)
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    Addgene inc teto-fuw-nicd fragment
    Loss of p 53 enhances the tumor-initiating potential of MM cells. (A) Growth curves of p 53-wt (Scr) and p 53-KO MM.1S, MM.1R, MOLP8, NCI-H929, and KMS11 cells (n = 3). (B) Primary (1°) and secondary (2°) colony formation of p 53-wt (Scr) and p 53-KO MM.1S, MOLP8, and NCI-H929 cells (n = 5). (C) Colony formation of p 53-wt (Scr) and p 53-KO MM.1R cells (n = 3). (D) Colony formation of p 53-null KMS11 (Ctrl) and KMS11 p <t>53-overexpression</t> cells (n = 3). (E) Frequency of CD138 neg and ALDH + cells within Scr and p 53-KO MM.1S, MM.1R, and MOLP8 cell lines by flow cytometry (n = 6).
    Teto Fuw Nicd Fragment, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nicd+fragment/teto+fuw+nicd/pmc10368840-30-1-11
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    nicd cdna fragment (5456 to 7819 nucleotides; nm_001105721(1747-2531aa)  (Genechem)
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    Genechem nicd cdna fragment (5456 to 7819 nucleotides; nm_001105721(1747-2531aa)
    Regulation of Notch1 signaling significantly alters cell proliferation in normal adult rat brain. Immunostaining shows the increasing number of DCX+ cells on day 3 following TBI in <t>NICD-Ad</t> group, compared with sham-TBI ( A–D ) and NC-TBI ( E–H ) groups.
    Nicd Cdna Fragment (5456 To 7819 Nucleotides; Nm 001105721(1747 2531aa), supplied by Genechem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nicd+fragment/nicd+cdna+fragment++5456+to+7819+nucleotides++nm+001105721+1747+2531aa+/pmc05703017-29-3-16
    Average 90 stars, based on 1 article reviews
    nicd cdna fragment (5456 to 7819 nucleotides; nm_001105721(1747-2531aa) - by Bioz Stars, 2026-09
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    Image Search Results


    Loss of p 53 enhances the tumor-initiating potential of MM cells. (A) Growth curves of p 53-wt (Scr) and p 53-KO MM.1S, MM.1R, MOLP8, NCI-H929, and KMS11 cells (n = 3). (B) Primary (1°) and secondary (2°) colony formation of p 53-wt (Scr) and p 53-KO MM.1S, MOLP8, and NCI-H929 cells (n = 5). (C) Colony formation of p 53-wt (Scr) and p 53-KO MM.1R cells (n = 3). (D) Colony formation of p 53-null KMS11 (Ctrl) and KMS11 p 53-overexpression cells (n = 3). (E) Frequency of CD138 neg and ALDH + cells within Scr and p 53-KO MM.1S, MM.1R, and MOLP8 cell lines by flow cytometry (n = 6).

    Journal: Blood Advances

    Article Title: Loss of p 53 enhances the tumor-initiating potential and drug resistance of clonogenic multiple myeloma cells

    doi: 10.1182/bloodadvances.2022009387

    Figure Lengend Snippet: Loss of p 53 enhances the tumor-initiating potential of MM cells. (A) Growth curves of p 53-wt (Scr) and p 53-KO MM.1S, MM.1R, MOLP8, NCI-H929, and KMS11 cells (n = 3). (B) Primary (1°) and secondary (2°) colony formation of p 53-wt (Scr) and p 53-KO MM.1S, MOLP8, and NCI-H929 cells (n = 5). (C) Colony formation of p 53-wt (Scr) and p 53-KO MM.1R cells (n = 3). (D) Colony formation of p 53-null KMS11 (Ctrl) and KMS11 p 53-overexpression cells (n = 3). (E) Frequency of CD138 neg and ALDH + cells within Scr and p 53-KO MM.1S, MM.1R, and MOLP8 cell lines by flow cytometry (n = 6).

    Article Snippet: For Notch intracellular domain (NICD) overexpression, the NICD fragment (from TetO-FUW-NICD; Addgene) was cloned into the pINDUCER21 (Addgene) lentiviral vector, then transfected into MM.1S p 53-KO cells.

    Techniques: Over Expression, Flow Cytometry

    Notch1-Hes1 signaling is required for increased clonogenic growth and drug resistance of p 53-KO MM TICs. (A) Colony formation of ID1-KD, ID2-KD, and nontargeting scramble (Ctrl)-treated p 53-wt (Scr) and p 53-KO MM.1S cells. (B) Western blot analysis of NOTCH1 and HES1 expression in p 53-wt (Scr) and p 53-KO MM.1S cells after treatment with a GSI. (C) Colony formation of HES1-KD and nontargeting scramble (Ctrl)-treated p 53-wt (Scr) and p 53-KO MM.1S cells (n = 4). (D) Colony formation of GSI-treated p 53-wt (Scr) and p 53-KO MM.1S, MM.1R, and MOLP8 cells (n = 4). (E) Western blot analysis of HES1 expression after doxycycline-induced Notch1 intracellular domain overexpression in p 53-KO MM.1S cells. (F) Colony formation of Notch1 intracellular domain overexpressing p 53-KO MM.1S cells after GSI treatment (n = 6). (G) Colony formation of GSI-treated clinical MM non-del17p samples (n = 6) and del17p samples (n = 3). (H) Colony formation of p 53-KO MM.1S cells after treatment with GSI (2.5 nM) and melphalan (Mel, 1 uM) or GSI and bortezomib (BTZ, 1.5 nM) (n = 5). (I) Melphalan IC 50 values of GSI-treated p 53-wt (Scr) and p 53-KO MM.1S cells (n = 3).

    Journal: Blood Advances

    Article Title: Loss of p 53 enhances the tumor-initiating potential and drug resistance of clonogenic multiple myeloma cells

    doi: 10.1182/bloodadvances.2022009387

    Figure Lengend Snippet: Notch1-Hes1 signaling is required for increased clonogenic growth and drug resistance of p 53-KO MM TICs. (A) Colony formation of ID1-KD, ID2-KD, and nontargeting scramble (Ctrl)-treated p 53-wt (Scr) and p 53-KO MM.1S cells. (B) Western blot analysis of NOTCH1 and HES1 expression in p 53-wt (Scr) and p 53-KO MM.1S cells after treatment with a GSI. (C) Colony formation of HES1-KD and nontargeting scramble (Ctrl)-treated p 53-wt (Scr) and p 53-KO MM.1S cells (n = 4). (D) Colony formation of GSI-treated p 53-wt (Scr) and p 53-KO MM.1S, MM.1R, and MOLP8 cells (n = 4). (E) Western blot analysis of HES1 expression after doxycycline-induced Notch1 intracellular domain overexpression in p 53-KO MM.1S cells. (F) Colony formation of Notch1 intracellular domain overexpressing p 53-KO MM.1S cells after GSI treatment (n = 6). (G) Colony formation of GSI-treated clinical MM non-del17p samples (n = 6) and del17p samples (n = 3). (H) Colony formation of p 53-KO MM.1S cells after treatment with GSI (2.5 nM) and melphalan (Mel, 1 uM) or GSI and bortezomib (BTZ, 1.5 nM) (n = 5). (I) Melphalan IC 50 values of GSI-treated p 53-wt (Scr) and p 53-KO MM.1S cells (n = 3).

    Article Snippet: For Notch intracellular domain (NICD) overexpression, the NICD fragment (from TetO-FUW-NICD; Addgene) was cloned into the pINDUCER21 (Addgene) lentiviral vector, then transfected into MM.1S p 53-KO cells.

    Techniques: Western Blot, Expressing, Over Expression

    Regulation of Notch1 signaling significantly alters cell proliferation in normal adult rat brain. Immunostaining shows the increasing number of DCX+ cells on day 3 following TBI in NICD-Ad group, compared with sham-TBI ( A–D ) and NC-TBI ( E–H ) groups.

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: Notch1 Signaling Activation Contributes to Adult Hippocampal Neurogenesis Following Traumatic Brain Injury

    doi: 10.12659/MSM.907160

    Figure Lengend Snippet: Regulation of Notch1 signaling significantly alters cell proliferation in normal adult rat brain. Immunostaining shows the increasing number of DCX+ cells on day 3 following TBI in NICD-Ad group, compared with sham-TBI ( A–D ) and NC-TBI ( E–H ) groups.

    Article Snippet: We obtained the NICD cDNA fragment (5456 to 7819 nucleotides; NM_001105721(1747-2531aa)) from the cDNA library of Genechem (Shanghai, China).

    Techniques: Immunostaining

    The alteration of spatial learning and memory in model rats after TBI. ( A ) The cognitive function of the experimental rats decreased significantly after injury. Compared to other groups, the NICD-Ad group recovered more quickly and the escape latency time significantly decreased (P<0.05). ( B ) The diagram represents the memory function through the time that the rats stayed in the target quarter during the probe trial. The results revealed the sham-Ad-TBI group in the target quarter was significantly higher than sham group and NICD-Ad group on day 7 after brain injury and the NICD-Ad group recovered on day 14 after brain injury. ( C ) The diagram also represents the memory function through the time spent searching for the platform in the target quarter. On day 1 after brain injury, the NICD-Ad group had the longest time spent searching in the target quarter. The rats treated with NICD-Ad had the shortest time needed to find the platform on day 3 after brain injury compared to the sham-Ad group rats (P<0.05).

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: Notch1 Signaling Activation Contributes to Adult Hippocampal Neurogenesis Following Traumatic Brain Injury

    doi: 10.12659/MSM.907160

    Figure Lengend Snippet: The alteration of spatial learning and memory in model rats after TBI. ( A ) The cognitive function of the experimental rats decreased significantly after injury. Compared to other groups, the NICD-Ad group recovered more quickly and the escape latency time significantly decreased (P<0.05). ( B ) The diagram represents the memory function through the time that the rats stayed in the target quarter during the probe trial. The results revealed the sham-Ad-TBI group in the target quarter was significantly higher than sham group and NICD-Ad group on day 7 after brain injury and the NICD-Ad group recovered on day 14 after brain injury. ( C ) The diagram also represents the memory function through the time spent searching for the platform in the target quarter. On day 1 after brain injury, the NICD-Ad group had the longest time spent searching in the target quarter. The rats treated with NICD-Ad had the shortest time needed to find the platform on day 3 after brain injury compared to the sham-Ad group rats (P<0.05).

    Article Snippet: We obtained the NICD cDNA fragment (5456 to 7819 nucleotides; NM_001105721(1747-2531aa)) from the cDNA library of Genechem (Shanghai, China).

    Techniques: